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首页> 外文OA文献 >Molecular cloning of two west African human immunodeficiency virus type 2 isolates that replicate well in macrophages: a Gambian isolate, from a patient with neurologic acquired immunodeficiency syndrome, and a highly divergent Ghanian isolate.
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Molecular cloning of two west African human immunodeficiency virus type 2 isolates that replicate well in macrophages: a Gambian isolate, from a patient with neurologic acquired immunodeficiency syndrome, and a highly divergent Ghanian isolate.

机译：在巨噬细胞中复制良好的两种西非人类免疫缺陷病毒2型分离株的分子克隆：来自神经系统获得性免疫缺陷综合症患者的冈比亚分离株和高度分化的加纳分离株。

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Human immunodeficiency virus type 2 (HIV-2)-related viruses were isolated from a Gambian dying of exclusively neurological disease (HIV-2D194) and from an asymptomatic Ghanian (HIV-2D205). Both strains exhibited properties of HIV-1 biological subtype c: they grew slowly and induced few or no syncytia but eventually produced high levels of particle-associated reverse transcriptase in cultures of fresh peripheral blood lymphocytes, and they established stable infection of T-lymphoma (HUT-78) and monocytic (U937) cell lines. Each produced even higher levels of reverse transcriptase when fresh human monocytes/macrophages were used as target cells. The viruses were molecularly cloned after a single passage in culture, in order to minimize in vitro selection of subtypes present in vivo. Restriction-site analysis showed heterogeneity within each isolate. Nucleotide sequence analysis of a portion of the HIV-2D194 genome revealed that it is a member of the prototypic HIV-2 family, displaying 13% divergence versus HIV-2ROD and HIV-2NIHZ, as compared to 9% divergence between HIV-2ROD and HIV-2NIHZ. In contrast, HIV-2D205 is the most highly divergent HIV-2 strain yet described: it is equidistant in relation between the known HIV-2 strains and the simian immunodeficiency virus isolates from rhesus macaque monkeys (23-25% divergence).
机译：与人类免疫缺陷病毒2型（HIV-2）相关的病毒是从仅因神经疾病而死的冈比亚（HIV-2D194）和无症状的加纳人（HIV-2D205）中分离出来的。两种菌株均表现出HIV-1生物亚型c的特性：它们生长缓慢且几乎不引起合胞体，但最终在新鲜的外周血淋巴细胞培养物中产生了高水平的颗粒相关逆转录酶，并且它们建立了T淋巴瘤的稳定感染（ HUT-78）和单核细胞（U937）细胞系。当将新鲜的人单核细胞/巨噬细胞用作靶细胞时，每种都产生更高水平的逆转录酶。为了使活体内存在的亚型的体外选择减至最少，病毒在进行单次传代培养后就进行了分子克隆。限制性酶切位点分析显示每个分离株内的异质性。对一部分HIV-2D194基因组的核苷酸序列分析表明，它是原型HIV-2家族的成员，与HIV-2ROD和HIV-2NIHZ相比显示出13％的差异，而在HIV-2ROD与HIV-2ROD和HIV-2ROD之间显示出9％的差异。 HIV-2NIHZ。相比之下，HIV-2D205是迄今描述的差异最大的HIV-2病毒株：在已知的HIV-2病毒株与猕猴的猿猴免疫缺陷病毒分离株之间的距离是等距的（23-25％的差异）。

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